Abstract: To establish UPLC fingerprint of Ultra-micro and Normal powder of Pueraria Radix respectively and compare the fingerprint.The UPLC chromatographic column was Acquity UPLC BEH C₁₈（50 mm×2.1mm，1.7 μm）.The mobile phase was acetonitrile-0.1% phosphoric acid water with gradient elution wavelength was 230nm and column temperature was 30 ℃ with the flow rate of 0.2 mL/min.The fingerprints of 10 batches of Ultra-micro and 10 batches of Normal were analyzed with 3′-hydroxy puerarin,puerarin,3′-methoxy puerarin,daidzin,genistin and daidzein the similarity analysis was carried out.The results of methodological study met technical requirements of UPLC fingerprint.The UPLC fingerprints of ultra –micro and normal powder of Pueraria Radix had obvious difference in area of common peaks but less differences in number of common peaks.The UPLC method was stable and accurate and could reflect the whole chemical composition information and provide a basis for the establishment of quality standard for Ultra-micro and Normal powder of Pueraria Radix.

Key words: Pueraria Radix, ultrafine powder, puerarin, Ultra-high performance liquid chromatography, chromatographic fingerprint, similariy analysis