Abstract: This study observed the effects of Tongfeng Kangning Formula on blood uric acid and serum XOD,ADA,IL-6,IL-8 and CRP in rats with hyperuricemia and acute gouty arthritis (AGA),evaluate the therapeutic effect of Tongfeng Kangning Formula and explore its action mechanisms.56 Wistar rats were randomly divided into 7 groups with 8 rats in each group:normal control group,model control group,the colchicine group,Tongfeng Kangning Formula groups of low,medium and high dose.Xanthine combined with intraperitoneal injection of oxazine acid potassium were used to establish rat model of hyperuricemia,and ankle cavity injection of uric acid sodium to used to establish AGA rat model.During the experiment,the degree of joint swelling in rats was observed before and after the drug intervention.The pathologic changes of ankle joint were observed via HE staining and ELISA was used to detect the contents of uric acid,XOD,ADA,IL-6,IL-8 and CRP in serum.Compared with model control group,the contents of blood uric acid an serum XOD,ADA,IL-6,IL-8 and CRP in rats of the Tongfeng Kangning Formula groups (P<0.05),and the conditions regarding inflammatory cell infiltration and synovial hyperplasia were improved.Tongfeng Kangning Formula has significant anti-effect on hyperuricemia and acute gouty arthritis,and its therapeutic mechanism may be related to the inhibition of XOD and ADA activity and the decrease of serum IL-6,IL-8 and CRP.

Key words: Tongfeng Kangning Formula, acute gouty arthritis, hyperuricemia, XOD, ADA, IL-6, IL-8, CRP