Abstract: To explore the effect of Sijunzi pill (SJZP) on the expression of substance P (SP) in jejunum,the level of mRNA and intestinal propulsive rate in spleen-qi deficiency rats,24 male spleen-qi deficiency SD rats were induced and randomly divided into the model group,SJZP group and the control group (8 per group).Carbon propelling was applied to observe the intestinal propulsion rate.Enzyme-linked immuno sorbent assay (ELISA) was applied to observe the SP in jejunum and the expression of mRNA level with reverse transcription-polymerase chain reaction (RT-PCR).In the SJZP treatment group,the SP and mRNA of spleen-qi deficiency rats were both increasing significantly (P<0.05).The small intestinal propulsion rate in SJZP group was higher than that of the control group.Compared with the control group,the body weight,diet and drinking amount in spleen-qi deficiency group increased greatly (P<0.05).The decrease of SP and mRNA in the mesenteric plexus of jejunum of rat model had some relationship with the spleen-qi deficiency.The regulation on SP and mRNA were the mechanisms of SJZP for spleen-qi deficiency.

Key words: spleen-qi deficiency, Sijunzi pill, substance P, jejunum, small intestinal propulsion rate