Abstract: To investigate the protective effect of isoquercitrin on t-BHP-induced oxidative damage of L-02 cells. In vitro oxidative stress damage model was established by treating L-02 cells with t-BHP(100 μmol/L) for 24 h.Cells were divided into control case,model case(100 μmol/L t-BHP),positive control case(10 μmol/L Vitamin E,VE) and isoquercitrin(1,10 and 100 μmol/L) cases.Cell viability was determined by MTT assay.Contents of LDH,ROS,MDA,GSH-Px and SOD activities were detected by ELISA methods.The protein expressions of sirtuin 6(Sirt6) and nuclear factor-k-gene binding p65(NF-κBp65) were determined by Western blot technology.Compared with model case,isoquercitrin significantly enhanced cell viability,GSH-Px and SOD activities,and decreased LDH activity,contents of ROS and MDA.Western blot showed isoquercitrin upregulated the expression of Sirt6 and downregulated the expression of NFκBp65.Isoquercitrin showed the protective effect on t-BHP-induced oxidative damage of L-02 cells and its protective effects may be related with increasing in ROS removal ability and regulation of Sirt6 and NF-κBp65 level.

Key words: isoquercitrin, L-02 cells, oxidative damage