Abstract: This study is to investigate the effect and mechanism of Zishenqinggan prescription (ZSQG) on the treatment of type 2 diabetes,and lay the foundation for its clinical application and further development.T2DM model rats were randomly divided into model group,metformin group,high,middle,low dose of ZSQG group.The effect of ZSQG on body weight,food intake,water intake,urine,stool,blood glucose,OGTT,HbAlc,C-peptide,GLP-1content,GSP,insulin of rats was studied;Western blot was used to detect AKT,p-AKT,GSK-3β,p-GSK-3β expression in liver tissue and to detect p-IRS-1,IRS-1,Glut4 expression in skeletal muscle tissue.ZSQG can effectively regulate sugar metabolic disorders in type 2 diabetic model rat,improve insulin resistance;enhance insulin secretion,regulate skeletal muscle PI3K/AKT,regulate liver AKT/GSK-3β signaling pathway,which may be the mechanism of ZSQG for the treatment of type 2 diabetes and to improve insulin resistance.

Key words: Zishenqinggan prescription, type 2 diabetes mellitus, effect, mechanism