Abstract: HPLC method was developed for the simultaneous determination of chlorogenic acid,vanililic acid,ellagic acid, sioquercitin, afzelin, quercetin, kaempferol, kaempferidel and ursolic acid in Cyclocaryae Folium.The ZORBAX Eclipse XDB-C₁₈ column (150 mm×4.6 mm,3.5 μm) was used with 0.2% phosphoric acid in acetonitrile (A) and 02% phosphoric acid in water (B) as mobile phases in gradient elution mode.The flow rate was 0.7 mL/min,the column temperature was 35 ℃.The DAD detection wavelength was set at 210 nm for vanililic acid,ellagic acid and ursolic acid,360 nm for the others.As a result,nine chemical markers were separated well,the average recoveries were between 9586-103.30%,RSD<2.5%.The developed HPLC method was simple,accurate and reproductive.It is suitable for the determination of nine chemical markers of Cyclocaryae Folium.

Key words: Cyclocaryae Folium, HPLC, multiple chemical marks, content determination