Abstract: Ultra-high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry (UPLC/Q-TOF-MS) combined UPLC and Q/TOF-MS organically.Due to the high separation ability and the high sensitivity of UPLC,the high resolution of Q/TOF-MS,UPLC/Q-TOF-MS was more and more extensive application in pharmaceutical analysis.This paper reviewed the applicability of UPLC/Q-TOF-MS in analyze the components of natural product,studies on pharmacokinetics,establishes TCM fingerprint and rapid identification of target components in recent years.We finally point out the advantages and perspectives of UPLC/Q-TOF-MS in the field of pharmaceutical analysis.

Key words: UPLC/Q-TOF-MS, pharmaceutical analysis, pharmacokinetics, fingerprints, rapid analysis