Abstract: To investigate the effect of luteolin on PTEN-PI3K-AKT signal pathway and immune function in lung cancer model rats.The rats were given disposable infusion of carcinogenic iodized oil via left lobe bronchus to induce lung cancer model.The rats were treated with high,medium and low dose of luteolin and cyclophosphamide respectively for 16 weeks.The general condition,histopathology and lung index,spleen index were evaluated.The expression of EGF,TGF-β and VEGF was detected by enzyme-linked immunosorbent assay (ELISA).The CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺  in T lymphocytes of rats were detected by flow cytometry.The expression of AKT-1,CyclinD1 and NF-κB mRNA in each group were detected by Real-time fluorescent quantitative PCR.The expression of PTEN,p-PI3K and p-AKT protein was detected by Western blot.The results showed that luteolin can significantly improve the pathology of lung tissue,decrease the lung index and increase spleen index.Luteolin cannot only reduce the levels of EGF,TGF-β,VEGF and AKT-1,CyclinD1,NF-κB mRNA and P-PI3K and p-AKT protein in lung tissue and the expression of CD8⁺ levels in blood,but also increase the expression of CD3⁺、CD4⁺、CD4⁺/CD8⁺ levels in blood and PTEN protein in lung tissue.Luteolin can significantly improve the immune function and downregulate the activity of PI3K/AKT signaling pathway in the Lung Cancer Model Rats.

Key words: luteolin, lung cancer, lung index, spleen index, PTEN-PI3K-AKT