Abstract: This paper studied the protective effect and mechanism of Curcumin (Cur) on exercised-induced renal slit diaphragm injury of rats.44 SPF Sprague-Dawley (SD) rats were randomly divided into three groups:control group (C),overtraining group (OT) and curcumin + overtraining group (COT).Rats in OT and COT group were trained for 6-week increasing load treadmill,and COT group were given 200 mg/kg/d (5 mL/kg) curcumin using intragastric administration,and other groups intervened the same volume 1% carboxymethyl cellulose.The pathological changes of renal tissues were observed after the last training of 24 h and detected the biochemical indicators of urine,blood and kidney tissues.The results showed that after 6-week treadmill training,the kidney tissues of rats in group C were normal,the pathological changes occurred in both OT and COT group and that of COT group were less than the OT group.The Paller scores of renal tubules was significantly higher than that of C group (P<0.01),and COT group were significantly lower than the OT group (P<0.05).Urinary total protein (TP),OT group was significantly higher than C group (P<0.01),and COT group was lower than OT group,but no significant difference (P> 0.05).Microalbumin (mAlb),OT group was significantly higher than C group (P<0.01),and the COT group was lower than OT group,but no significant difference (P> 0.05).Serum testosterone (T),OT group was lower than C group (P<0.01),and COT group was significantly higher than OT group (P<0.01).Corticosterone (C),OT group was significantly higher than that of C group (P<0.01),and COT group was significantly lower than OT group (P<0.01).The change trend of T/C between groups is consistent with T.Serum creatinine (SCr)and Blood urea nitrogen (BUN),OT group were significantly higher than C group (P<0.01),and COT group was significantly lower than OT (P<0.01).The expression of Nephrin in kidney was significantly lower in OT group than C group (P<0.01),and COT group was significantly higher than OT group (P<0.05).Renal NF-κB p65 protein expression,OT group was higher than C group (P<0.01),and COT group was significantly lower than OT group (P<0.05).Renal Tumour necrosis factor-α(TNF-α),OT group was significantly higher than C group (P<0.01),and COT group was significantly lower than OT group (P<0.05).AngiotensinⅡ AngⅡn,OT group was significantly higher than C group (P<0.01),COT significantly below OT group (P<0.05).The results showed that 6-week intensive treadmill training resulted in overtraining and renal slit diaphragm injury of rats.Curcumin effectively prevent the happening and development of overtraining by inhibiting the expression of the NF-κB,reducing TNF-α and the activity of RAS,alleviateing the expression of Nephrin,thus protect the structure and function of renal slit diaphragm in rats.

Key words: Curcumin, exercised-induced renal injury, slit diaphragm, NF-κB, Nephrin