Abstract: Seven compounds were isolated from the 95% ethanol extract of Ganoderma sinense.Their structures were characterized as Ganosinensic acid C (1),Lucidenic acid A (2),Lucidone B (3),Ganoderenic acid B (4),Ganoderenic acid E (5),Ganoderic acid H (6) and Ganoderic acid I (7).Compound 1 was a new natural product by means of 2D-NMR and HR-ESI-MS experiments.MTT assays were performed to detect the viability of DU145、CEM and Hela cells by different concentrations of 1 Compound 1 showed cytotoxic activities with IC₅₀ values of 28.8±1.5 μMol/L against Hela cells.

Key words: Ganoderma lucidum, ganoderma sinense, ganosinensic acid C, triterpenoids, chemical constituents, cytotoxicity