Abstract: The determination method of panaxadiol in Ginseng hydrolysate was established by HPLC-ELSD.The optimal hydrolysis condition of panaxadiol was investigated.For HPLC-ELSD,an Agilent ZORBAX SB-C₁₈ column (4.6 mm×150 mm,5 μm) was applied,the mobile phase consisted of acetonitrile-water (75∶25),Hybrid method:offline hybrid,the flow rate was 0.8 mL/min,the column temperature was 30 ℃,the drift tube temperature of ELSD was set at 85 ℃,and the flow rate of air was 2.6 mL/min.Based on panaxadiol content,the hydrolysis process was optimized from three aspects:the amount of hydrochloric acid,the hydrolysis temperature and the hydrolysis time.According to the regression equation,it was found that as for panaxadiol,the linear detection range was 0.494-1.978 μg (R²=0.9998),the recovery was from 98.79%-101.37%,and the relative standard deviation was 1.07% (n=6).Under the determination method,the optimal hydrolysis conditions were:acid concentration control at 5%,hydrolysis temperature 80 ℃,and time was 3 h.In conclusion,a HPLC-ELSD method for determination of panaxadiol in Ginseng was established for the first time,the method was reliable and easy.The optimized process provided scientific basis for the comprehensive exploitation and utilization of panaxadiol.

Key words: ginseng, panaxadiol, hydrolysis, HPLC-ELSD