Abstract: To explore the effect of tetrahydrocorysamine on the apoptosis of Alzheimers disease (AD) cell model and its possible mechanism.Alzheimer's disease cell model was established by Aβ_25-35 inducing PC-12 cells.Flow cytometry was used to measure the apoptosis rate,high-throughput and high-content analysis system was used to observe the nuclear morphological changes and detect mitochondrial membrane potential (MMP) changes,the expression of apoptosis-related protein was detected by Western blot.The results showed that SQZJSM can improve the damaged nuclear morphology of model cells,and significantly reduce the apoptosis rate and Cyt C level in the cytoplasm,up-regulate the level of MMP,decrease the expression of Bax,Cleaved-Caspase3,Cleaved-Caspase 9 protein,and increase the level of Bcl-2,p-Akt/T-Akt (P <0.05);PI3K/AKT inhibitor LY294002 can block the improvement of SQZJSM on model cells.The above results indicate that tetrahydrocorysamine can significantly reduce the apoptosis rate of AD cell model induced by Aβ_25-35,which may be related to the activation of PI3K/Akt signaling pathway to regulate endogenous mitochondrial apoptosis.

Key words: corydalis, tetrahydrocorysamine, Aβ_25-35, PC-12, mitochondrial apoptosis, Akt