Abstract: To establish a HPLC method for the simultaneous determination of Luteolin,6,7-dimethoxycoumarin,Scopoletin,5,7-dimethoxy coumarin,7-hydroxy coumarin，Hesperidin,Quercetin,Ferulic acid in Fructus ctris sarcodactylis from different regions.Thermo BDS HYPERSILC₁₈ (250 mm× 4.6 mm,5 μm) column was used，with methanol-0.2% glacial acetic acid as the mobile phases in gradient elution mode.The column temperature was 30 ℃,with flow rate 1.0 mL/min.The detection wavelength was set at 322 nm.The eight kinds of active ingredients components in Fructus ctris sarcodactylis were separated well in the chromatographic conditions.The average recoveries were between 91.22%-96.52%,RSD<5.0%.There were differences in the contents of eight components in 48 species.There was a correlation between some active ingredients.The samples of medicinal materials from 48 producing area could be divided into 3 categories.The quality of Fructus ctris sarcodactylis from Lao weng town,changning county,yibin city,sichuan province was the best.This study on active ingredients in Fructus ctris sarcodactylis can be used to provide guidance for the quality control and safety evaluation．

Key words: Fructus ctris sarcodactylis, HPLC, determination, correlation analysis, principal component analysis, cluster analysis