Abstract: In order to investigate the effects of tenuifolin (TEN) in the induction of SH-SY5Y cell oxidative damage induced by Aβ_25-35 and the underlying mechanisms.Aβ_25-35-induced cell injury models were established.Interferon TEN and autophagy inhibitor 3-MA were intervened.The morphological changes were observed by microscopy.The oxidative stress levels were detected by kits.RT-PCR and Western blot were used to detect the changes in Beclin-1,LC3,mTOR,AMPK and ULK1 mRNA and protein levels before and after TEN and 3-MA.It was found that TEN improved the morphological damage and cell viability of SH-SY5Y cells induced by Aβ_25-35.Compared with Aβ_25-35 group,TEN decreased the concentration of ROS and MDA,and increased the activity of SOD,GSH-Px and CAT.The expression levels of LC3-II/I and Beclin-1 increased in Aβ_25-35 group,and it's further increased in TEN group.And TEN increased the expression of AMPK and ULK1,decreased the expression of mTOR.The addition of 3-MA antagonizes the action of TEN.However,3-MA reversed the effects of TEN.In conclusion,it can be proved thatTEN may activate autophagy by regulating AMPK/ mTOR/ ULK1 pathway to inhibit the cell oxidative damage induced by Aβ_25-35,and play a neuroprotective role.

Key words: tenuifolin, Alzheimer's disease, &beta, -amyloid protein 25-35, autophagy, oxidative stress