Abstract:

Human glioma U87 cells were treated with curcumin and the effect on cell proliferation was assayed by CCK-8 method,the morphological changes were observed NADPH oxidase in cells were tested by ELISA.DCFH-DA fluorescence stain was used and the cells ROS content was detected.The oxidative stress indexes total antioxidant capacity(T-AOC),malondialdehyde(MDA),superoxide dismutase(SOD) and glutathione(GSH) were tested.Apoptosis was analyzed by AO/EB fluorescence staining and flowcytometry after double staining with Annexinv/PI.Finally,apoptosis-related signaling pathway protein was detected by immunoblotting.The results showed that curcumin promoted ROS production by increasing cell NADPH oxidase activity,which decreased intracellular T-AOC,increased MDA content,decreased GSH content,and increased SOD activity.These indicated curcmin induced oxidative stress in cells,and possibly increased ROS to trigger the cell signaling pathway by down-regulating the expression of NF-κB/p65 protein,and finally promoted apoptosis through the apoptosis-executing molecule Caspase-3.

Key words: curcumin, reactive oxygen species, oxidative stress, apoptosis