To investigate the immunomodulatory effects of polysaccharide from Oudemansiella furfuracea on murine macrophages in vivo and in vitro.Peritoneal macrophages were collected using peritoneal lavage method and the culture system was established.Chick red blood cell phagocytosis test,fluorescent probe technique,total nitric oxide assay and enzyme-linked immunosorbent assay were carried out to measure phagocytic activity,the content of nitric oxide,the levels of tumor necrosis factor-α,interleukin-1,interleukin-6 and interleukin-12 in culture supernatant,respectively.The results showed that polysaccharide from O. furfuracea in the range of certain concentration enhanced the phagocytosis ability and NO content of murine macrophages in vivo and cultured in vitro,and improved the secretion level of tumor necrosis factor-α,interleukin-1,interleukin-6 and interleukin-12 in culture supernatant,compared with control group.So,the immune function of murine macrophages could be modulated by polysaccharide from O. furfuracea at a certain concentration.