This paper investigated the chemical constituents from a valuable traditional Chinese medicine,Balanophora harlandii, collected in Meizhou,Guangdong Province,as well as the secondary metabolites from the culture broth of its endophytic fungus Penicillium coprophilum Mzz9.As a result,eleven compounds were isolated and identified by combining various chromatographic and spectroscopic techniques.Among them,five compounds including lupeol (1),β-amyrin (2),clearosterol (3),lupeol acetate (4) and glycerin linoleate (5) were isolated from the dichloromethane extracts of Balanophora harlandii,while the other six compounds,including dechlorinated griseofulvin (6),griseofulvin (7),dehydrogriseofulvin (8),oxaline (9),4-megastigmen-3,9-dione (10),and p-hydroxyacetophenone (11),were purified and identified from the culture broth of P. coprophilum Mzz9. Compounds 1 and 5 were isolated from B. harlandii for the first time,while compounds 6-11 were identified for the first time from the endophytic fungus of B. harlandii.