Determine astragaloside A and isoflavone glucoside in local Astragali Radix in Ningxia by HPLC,collect the MIR spectrum of Astragali Radix and combined partial least squares (PLS) to establish a MIR quantitative analysis model for astragaloside A and isoflavone glucoside in Astragali Radix,and verify with correction set. At the same time,to analyzed and evaluate the infrared spectrum and second derivative spectrum of Astragali Radix,the infrared spectrum was analyzed by cluster analysis and principal component analysis. The best model of astragaloside in the middle infrared spectrum after second derivative treatment,the model parameter R² is 99.72,SEE is 0.003 7,SEP is 0.004 2. The best model was obtained after the second derivative treatment of the infrared spectrum of the isoflavone glucoside,the model parameter R² is 99.33,SEE is 0.000 6,SEP is 0.000 7. The similarity coefficients of Astragali Radix in different regions were all above 0.90;But the components of ester,cellulose and starch in Astragali Radix were different in the second derivative spectra. Cluster analysis and principal component analysis showed that there were obvious differences between Tongxin and other regions. The collective results provide that the model used for quantitative analysis of astragaloside A and isoflavone glucoside in Astragali Radix in Ningxia has certain reliability and prediction ability. The quality of Astragali Radix membranaceus in Ningxia can be evaluated by infrared spectroscopy.