In order to obtain the high purity flavonoid and seamier composition Ginkgo leaves flavonoids extract (GBFE) from Ginkgo leaves extract (GBE).The UV spectrophotometer total flavonoids test method was built with 2″-(6″-p-coumaroylglucosyl) quercitrin(CGQ) as standard;Though twice polyamide column tomography process,the total flavonoids of GBFE is more than 92%.During the twice polyamide column method screened,Na₂CO₃ and ethanol solutions were chosen as eluent.And most impurities were separated with flavonoids.In this process,the total flavonoids recovery ratio is more than 60%,and similarity with GBE of flavonoids part.By the pilot experiments,the process is stability and suitable for production.