The isolation and purification methods of the main components from Polygonum cuspidatum were established and the activity of the main components on anti-hyperuricemia was investigated.The microporous resin combined with high speed current chromatography (HSCCC) were used to separate and purify the three compounds,namely polydatin,resveratrol and emodin.The hyperuricemia of rat model was made by oxonic acid potassium salt.The level of uric acid (UA),creatinine (Cr) and blood urea nitrogen (BUN) in rat serum were measured.The results showed that polydatin and resveratrol were separated by macroporous resin and emodin by HSCCC.The levels of UA,Cr,BUN of the hyperuricemia of rat model were increased comparing to the normal rats.Based on the hyperuricemia of rat model,polydatin,resveratrol and emodin significantly reduced the level of UA.In conclusion,polydatin, resveratrol and emodin from P. cuspidatum were rapidly separated and purified by macroporous resin and HSCCC,and the three main compounds exhibited an obviously positive effect on hyperuricemia rat.