The objective of this paper is to investigate the therapeutic effects of extract of Yunnan Diaphragma Juglandis Fructus (YN-DJF) on hyperuricemia (HUA) models in mice.The HUA models in mice were established by i.g. oxygen oxazine acid potassium and adenine,and the therapeutic effect of 4 YN-DJF extracts,including water extract,alcohol extract,and the water extracts with two different deionization treatments,were evaluated.After 14 days of continuous administration,the contents of UA,Cr,and BUN in serum,the XOD activity of liver tissue,and the level of IL-1β and TNF-α in kidney were tested,and the pathological structure changes of renal tissue were observed.The results showed that,compared with the model groups,4 YN-DJF extracts performed different therapeutic levels by reducing the content of UA,Cr,and BUN,suppressing XOD activity,reducing IL-1β and TNF-α level,and alleviating pathological structural changes.Alcohol extract of YN-DJF was the most effective among all extracts.It proved that extracts of YN-DJF could be applied to treat HUA mouse by improving renal function,suppressing XOD activity,and anti-inflammatory effects.