NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2021, Vol. 33 ›› Issue (9): 1519-1526. doi: 10.16333/j.1001-6880.2021.9.010

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Isolation and purification of a novel peptide from the fruiting bodies of Morchella importuna and its neuroprotective effect

XIONG Chuan1,HUANG Wen-li1,JIN Xin1,LUO Qiang2,LI Ping1,ZHANG Lu1,LI Qiang3,ZHU Yu1*   

  1. 1Biotechnology and Nuclear Technology Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610061,China; 2Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education),Institute for Viral Hepatitis,Department of Infectious Diseases,the Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China; 3College of Pharmacy and Biological Engineering,Chengdu University,Chengdu 610106,China

  • Online:2021-09-28 Published:2021-09-28

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Abstract:

In this research,we isolated and purified a novel peptide (MIP-16) from the fruiting bodies of Morchella importuna M.Kuo.MIP-16 was extracted with phosphate buffer,and purified by molecular exclusion chromatography followed by reversed-phase high performance liquid chromatography (RP-HPLC).The amino acid sequence of MIP-16 was determined by LC-MS-MS as Thr-Ile-Thr-Leu-Glu-Val-Glu-Ser-Ser-Asn-Ile-Thr-Asn-Asp-Val-Lys with molecular mass of 1 762 Da.6-hydroxydopamine-induced apoptotic PC12 cell model was used to verify the protective effect of MIP-16 on PC12 cells from apoptosis.The results showed that treatment with MIP-16 significantly inhibits the production of malondialdehyde (MDA),reduces the level of reactive oxygen species (ROS),regulates the ratio of Bcl-2 and Bax,and reduces the activity of Caspase in PC12 cells;indicating its neuroprotective activity through a mitochondrial pathway.These results suggest a potential application of MIP-16 as an adjuvant therapy for the treatment of Parkinson's syndrome.

Key words: Morchella importuna, peptide, apoptosis, neuroprotective activity

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