Plumeria contains rich functional compounds.In this study, 2-NBDG glucose uptake kit was used to measure cell fluorescence intensity under different concentrations of Plumeria  water extract (PE),and the phosphorylation level of Akt and the expression of other key factors in C2C12 skeletal muscle cells were detected by Western blot.The results showed that the glucose intake of C2C12 skeletal muscle cells after PE treatment was significantly increased compared with the blank group.After C2C12 skeletal muscle cells were cultured in the medium containing 4 mg/mL PE for 30 min,the phosphorylation level of Akt was significantly increased.Meanwhile,the extract did not up-regulate the phosphorylation of AMPK Thr172,suggesting that the increase of glucose uptake by PE in C2C12 skeletal muscle cells was closely related to the phosphorylation of Akt.The composition of PE was characterized by GC-MS,and the composition and content of PE were determined.Through the analysis of the related components of hypoglycemic,it was concluded that the regulation of glucose transport in PE might be related to some components in Plumeria ,which one or many are still to be explored.