The aim of this study is to explore the molecular mechanism of the total alkaloids of Sophora alopecuroides(TASA) extracted from the aboveground part of S.alopecuroides on the hypoglycemic effect of L6 skeletal muscle cells.TASA was found to increase glucose uptake in L6 cells using glucose detection kit,and MTT results showed that TASA had no cytotoxicity on L6 cells.TASA could promote the translocation of GLUT4 by laser confocal microscopy.Western blot results showed that TASA could promote the expression of GLUT4 in L6 cells and enhanced the phosphorylation of AMPK and PKC.These results suggested that TASA could promote the expression and transport of GLUT4 through AMPK and PKC signaling pathways,and then promote glucose uptake,suggesting that TASA may have the potential to be developed as a novel hypoglycemic drug.