Sargentodoxa cuneata extracts possessed anti-inflammatory,antioxidant,and neuroprotective effects.Herein,a phenylpropanoid sargentol from this plant was studied to evaluate its neuroprotective effect using hydrogen peroxide (H₂O₂),rotenone,sodium azide (NaN₃) induced damage in PC12 cell,and the cell viability was detected by MTT method.DCFH-DA probe was used to test the intracellular reactive oxygen species (ROS) level.AutoDock Vina was used to dock sargentol with 14 selected target proteins,to virtually screen the potential target according to the docking scores.The pretreatment of PC12 cell with sargentol (0.1,1,10 μmol/L) obviously increased cell viability damaged by H₂O₂ (P< 0.05) and inhibited the ROS level.While sargentol had moderate protective effect against rotenone injured PC12 cell (P> 0.05),only had slight effect on NaN₃ injured PC12 cell(P> 0.05).Sargentol had higher binding affinity with proteins 5D3I,2Y37,1E7V,4L7B and 3RZF,and possessed highest affinity of -7.7 kcal/mol with 5D3I (TLR2) and 2Y37 (iNOS).These findings suggested that sargentol might be serving as a novel potential resource for neuroprotection,which could be associated with ROS and inflammation.