In this paper,using donkey whey protein as the raw material,the DPPH radical scavenging rate was used as the index,using a computer simulation enzyme to desolve the donkey serey protein,the most suitable proteolytic enzymes capable of producing antioxidant active peptides were selected,using pH,enzyme lysis temperature,enzyme substate ratio (mass ratio) as the independent variable,using Design-Expert V8.0.6 to determine the best process conditions for the preparation of antioxidant peptide by-trypsinase.The results showed that at the substrate concentration of 4% and 4 h,the antioxidant activity was 4%,the enzyme was 4%,and the DPPH radical scavenging rate of 10 mg/mL donkey whey peptide was up to 46.23%.