HPLC fingerprint and chemical pattern recognition technology were used to evaluate the quality of Bombyx Batryticatus.Insertsil ODS-3 column (4.6 mm×250 mm,5 μm) was used for gradient elution with acetonitrile-0.1% phosphoric acid solution as mobile phase.The detection wavelength was 365 nm,and flow rate was 0.8 mL/min.The injection volume was 10 μL,and column temperature was 30 ℃.Similarity evaluation,Cluster analysis(CA),and principal component analysis(PCA),etc,were used to analyze and evaluate the quality of 20 batches of samples from different areas.Nine common peaks were confirmed in the fingerprint.Five chromatographic peaks were identified,rutin,hyperin,rhodoside,quercetin and kaempferol.In addition,the correlation analysis were uesd between nine common peak areas and their appearance characters,total ash,acid-insoluble ash,extract and Beauverin content.The results showed that the HPLC fingerprint of Bombyx Batryticatus established in this study combined with chemical pattern recognition is simple and convenient,reliable,repeatable and specific,and has a certain correlation with the quality control indexes such as the character of medicinal materials,total ash,extract and Beauverin,which provides the basis and reference for the follow-up quality control of Bombyx Batryticatus.