In this study,the high performance liquid chromatography (HPLC) fingerprint was used to evaluate the quality of Aurantii Fructus from different habitats.Similarity evaluation,cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were adopted to analyze fingerprint data.The results showed that 11 batches of Aurantii Fructus have 16 common peaks with similarity ranged from 0.992 to 0.999,and the content of 13 chemical components has been determined.According to the cluster analysis and principal component analysis,the samples of Aurantii Fructus could be divided into four types.The comprehensive principal component analysis scores evaluated the quality of different batches of Aurantii Fructus.Orthogonal partial least squares method was used to screen out 4 components with quality differences,including naringin,narirutin,hesperidin and poncirin.In this study,the method combining the HPLC fingerprint of Aurantii Fructus with chemical measurement was simple and accurate,which could provide references for the quality control and evaluation of Aurantii Fructus.