In this study,HPLC was used to establish the characteristic chromatograms of different medicinal Chrysanthemi Flos species.The similarity evaluation of characteristic chromatograms,cluster analysis (CA),principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were combined,which was used to study the common components and differential components of 5 different Chrysanthemi Flos cultivars.21,20,25,22 and 22 characteristic peaks were determined from the characteristic chromatograms of Chrysanthemum morifolium (Ramat).Tzvel.‘Boju’ cv.nov.(BJ),C. morifolium (Ramat).Tzvel.‘Chuju’ cv.nov.(CJ),C. morifolium (Ramat).Tzvel.‘Gongju’ cv.nov.(GJ),C. morifolium (Ramat).Tzvel.‘Hangju’ cv.nov.(HAJ) and C. morifolium (Ramat).Tzvel.‘Huaiju’ cv.nov.(HUJ) respectively,17 characteristic peaks were determined from the characteristic chromatogram of Chrysanthemi Flos,and 12 chemical components were identified.The similarity of the five species was above 0.971,but the interspecific similarity was low.CA,PCA and OPLS-DA could accurately classify the five Chrysanthemi Flos species into five categories.Combined with OPLS-DA,Isochlorogenic acid B,3,5-O-diccoffeyl quinic acid,diosmetin,cryptochlorogenic acid,chlorogenic acid,neochlorogenic acid,luteolin,geranitin-7-O-β-D-glucoside and other 10 common components in Chrysanthemi Flos may be the feature markers of Chrysanthemi Flos.Apigenin,apigenin-7-O-β-D-glucoside,luteolin-7-O-glucoside and isochlorogenic acid C may be the feature markers that lead to the difference of Chrysanthemi Flos cultivars.HPLC characteristic chromatograms of Chrysanthemi Flos combined with chemometrics were used to screen out the common substance basis of medicinal Chrysanthemi Flos as feature markers and feature markers of significant differences among different varieties of Chrysanthemi Flos,which could provide scientific basis for overall quality evaluation,variety identification and single variety characteristic evaluation of Chrysanthemi Flos.