To explore the effect of banana peel extract on intestinal motility in constipated mice.In this study,the mice model of constipation was established with the mixed antibiotic solution.The effects of banana peel extract on intestinal flora,intestinal neurotransmitter and intestinal motiveness of constipated mice were measured.The results showed that the fecal water content increased by 137.5%,the intestinal propulsion rate increased by 3.4 times,and the first black stool excretion time decreased by 73.7% (P< 0.05) in the high-concentration banana peel extract group,which basically recovered to the level of the blank group.The phyla horizontal flora was disordered and some beneficial bacteria were missing in the constipation model mice.After the intervention of low concentration banana peel extract,the relative abundance of Trabulsiella and Unclassified-f-Enterobacteriaceae increased by 6 times,the relative abundance of intestinal Bacteroides decreased by 94.4%.Furthermore,the concentrations of norepinephrine,dopamine,gamma-aminobutyric acid and acetylcholine of neurotransmitters were significantly increased by 6.1%,17.6%,63.7% and 52.6%.These results indicated that banana peel extract could regulate intestinal flora and intestinal neurotransmitter levels to improve intestinal motility in constipated mice.