The distribution and population differences of endophytic fungi in Amomum villosum Lour. from Xishuangbanna and Maguan in Yunnan were studied,as well as their antioxidant and antibacterial potentials,in order to provide resources for the acquisition of new naturally active metabolites.Here,we isolated and purified endophytic fungi using plant tissue surface sterilization and identified them through ITS sequence analysis.Meanwhile,the activities of fermented endophytes culture extracts against five strains of bacteria were determined using filtering paper method,and antioxidant activity was evaluated by assessing the total reducing power,including scavenging activites based on DPPH radical and hydroxyl radical.As a result,a total of 77 strains of endophytic fungi were identified from A. villosum distributed in Xishuangbanna and Maguan,including 39 stains isolated from Xishuangbanna A. villosum,and 38 stains isolated from Maguan  A. villosum.The dominant genera in Xishuangbanna A. villosum were Penicillium,Colletotrichum,Xylaria,Daldinia, and Diaporthe,while the dominant genera in Maguan A. villosum were Penicillium, Phanerochaete,Pyrenochaetopsis,Neopestalotiopsis, and Cladosporium.Additionally,the Shannon diversity index,Simpson diversity index,and evenness of endophytic fungi in MaguanA. villosum were higher than those in Xishuangbanna A. villosum,and the Jaccard similarity coefficient between them was 0.222 2.Strains BSR 18,BSR 32,BSR 34,and MSR 15 showed higher antibacterial activity,while strains BSR 10 and BSR 18 had strong antioxidant activity.Our research preliminary discussed the diversity of endophytic fungi form Xaishuangbanna and Maguan,and furthermore screened the activty of endophytic fungi from A. villosum.There were significant differences and affluent diversity among endophytic fungi from A. villosum distributed in Xishuangbanna and Maguan.Therefore,strains with good antibacterial and antioxidant activities could be used as sources of natural active compounds for further study.