In order to comprehensively evaluate the phenolic components in Lycii Fructus,this study prepared non extractable phenolic compounds (NEPC) from Lycii Fructus after removing free polyphenols using alkaline hydrolysis and acid hydrolysis,respectively.The Box-Behnken experimental design method was selected to establish a mathematical model between NEPC yield and hydrolysis conditions,such as acid or alkali concentration,hydrolysis time,and material liquid ratio.The results of the response surface experimental design analysis indicated that in alkaline hydrolysis,the hydrolysis time has a significant impact on the yield of NEPC,but in acid hydrolysis,the ratio of material to liquid in acid hydrolysis has a considerable impact on the outcomes.Under the optimum circumstances,the yield of NEPC were 0.65±0.05 mg/100 g for the alkaline hydrolysis process and 0.52±0.03 mg/100 g for acid hydrolysis procedure,respectively.The chemical components in the hydrolysates were analyzed using UPLC-Q-TOF-MS.Eleven compounds were identified,which were the phenylpropanoids.In conclusion,the acid hydrolysate contained eleven compounds,while the alkali hydrolysis products had only four substances.Alkali hydrolysis produced more NEPC,whereas acid hydrolysate showed a good diversity of chemicals.Hence,different hydrolysis methods should be chosen for different research purposes while preparing NEPC.