This study aimed to investigate the effects of the fermentation broth of Eurotium cristatum on lipid metabolism and intestinal flora disorder in obese rats and provide the scientific basis for its development and utilization.Healthy SD rats were randomly divided into normal group,model group,low-dose,medium-dose and high-dose groups and Xuezhikang positive control group.The model of food-borne obese rats was established by high-fat feeding.The normal and model groups were gavaged with sterile water,and the intervention groups were gavaged with the fermentation broth of Eurotium cristatum.The experiment lasted for eight weeks and the body weight of the rats was measured and recorded weekly.After the last administration,feces and serum were collected for intestinal flora analysis and related biochemical indexes,and liver was extracted for pathological observation.The results showed that compared with the normal group,rats in the model group showed significant increases in body mass and Lee′s index,abnormalities in blood lipids,significant decreases in the diversity of intestinal flora.The fermentation broth of Eurotium cristatum significantly reduced the contents of total cholesterol,triglyceride and low density lipoprotein in serum of obese rats,increased the content of high density lipoprotein.Meanwhile,the sequencing results showed that the fermentation broth of Eurotium cristatum significantly increased the diversity of intestinal flora of rats,up-regulate the abundance of Ruminococcus NK4A214_group,UCG_005,Christensenellaceae_R-7_group,and down-regulate the abundance of Bacteroides and Turicibacter.LefSe analysis also showed that the fermentation broth of Eurotium cristatum could significantly increase the abundance of Lactobacillus and improve the intestinal defense function in rats.In conclusion,the fermentation broth of Eurotium cristatum could effectively improve lipid metabolism and intestinal flora disordesr in obese rats with high-fat diet.