Using the methods of animal model,plasma pharmacochemistry and metabolomics,the mechanism of intervention of Strychnine Semen before and after processing inflammation in rats was investigated.The toxicity before and after processing was preliminarily investigated according to the mortality of mice given different doses.The rat model of egg white foot swelling was established,and the anti-inflammatory effect was evaluated by swelling rate and inflammatory factor level before and after processing.High resolution mass spectrometry combined with multivariate statistics were used to identify the migratory components and their metabolites.The metabolic profiles and different biomarkers of each experimental group were compared and analyzed,and their metabolic pathways were constructed.The results showed that the toxicity of sand-scorch product at high dose was significantly lower than that of raw product.Both raw and sand-scorch products could significantly reduce the swelling rate and the levels of tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β) and interleukin-6 (IL-6) of rats′ feet,and there was no statistical difference in anti-inflammatory effect before and after processing (P>0.05).Through plasma drug analysis,12 transitional components and corresponding 60 metabolites were identified,and 10 different compounds were identified by S-plot.Metabolomics experiments found significant changes in the content of ten endogenous metabolites (P<0.05).The biomarkers mainly involved phenylalanine metabolism,glycerophospholipid metabolism,glutamic acid and glutamine cycle,arginine and proline metabolism and other pathways.This study confirmed that both raw and sand-scorch products of Strychnine Semen had good anti-inflammatory effects,and high temperature processing could reduce the toxicity of Strychnine Semen.After entering the blood,the active ingredients of the sand-scorch products should mostly be alkaloids,and they are easily eliminated by the body after I reaction.The difference analysis can indirectly prove that the anti-inflammatory effect of the sand-scorch products is higher than that of the raw products.The anti-inflammatory,analgesic and attenuated mechanisms of sand-scorch products are mainly related to amino acid metabolism and lipid metabolism.The experimental results further elucidated the active substance basis and the mechanism of action in vivo,and provided scientific support for the subsequent improvement of processing technology and the development of new drugs.