Based on the concept of "quantitative analysis of multi-components by single-marker",this study aims to establish a method for simultaneously determining the content of six cyclic terpenoid glycosides,namely morroniside,gardenoside,loganin,picroside II,picroside I,and harpagoside,in four medicinal materials:Gardeniae Fructus,Corni Fructus,Picrorhizae Rhizoma,and Scrophulariae Radix.The specificity of each iridoid glycoside in four medicinal herbs were determined using ultra-high performance liquid chromatography coupled with quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-Q-Exactive Orbitrap-MS) technology.For the high-performance liquid chromatography (HPLC) method,gardenoside served as the internal reference,and a multi-point calibration method was utilized to calculate the relative correction factors for five components to be tested,namely quantitative analysis of multi-components by single-marker (QAMS),morroniside,loganin,picroside II,picroside I,and harpagoside.A quantitative analysis of multi-components by QAMS method was established and compared with the measured values obtained using the external standard method (ESM).The relative correction factors for morroniside,loganin,picroside II,picroside I,and harpagoside were calculated using the multi-point calibration method,with values of 0.894 8,1.096 8,1.284 9,1.700 8 and 1.405 1,respectively.There was no significant difference in QAMS and ESM measurements,the relative standard deviation is less than 1.0%.Based on the concept of "quantitative analysis of multi-components by single-marker",a method for simultaneously determining the content of six cyclic terpenoid glycosides,including gardenoside in Gardeniae Fructus,morroniside and loganin in Corni Fructus,picroside II and picroside I in Picrorhizae Rhizoma,and harpagoside in Scrophulariae Radix,has been established.This method is accurate and feasible,and can be used for the quality evaluation of Gardeniae Fructus,Corni Fructus,Picrorhizae Rhizoma,and Scrophulariae Radix,which provides a reference for the quality evaluation of other medicinal materials.