The study investigated the effect of organic and inorganic fertilizers applied in combination on the yield and quality of Sichuan Angelica dahurica var. formosana in the hilly area of Sichuan Province, aiming to establish an optimal fertilization protocol for high quality and high yield cultivation in Suining City. A three-dimensional quadratic orthogonal rotation combination design was implemented, with regression models developed to analyze agronomic traits, yield, imperatorin, isoimperatorin content, and fertilizer interactions, supported by laboratory assays. Key findings revealed that: Appropriate organic fertilizer application significantly enhanced Angelica dahurica var. formosana yield; NPK fertilizers promoted dry matter accumulation; Micronutrient fertilizers effectively regulated imperatorin and isoimperatorin biosynthesis. hrough comprehensive evaluation of agronomic parameters, yield, and active compound dynamics under varying fertilization regimes, the optimal high-quality, high-yield fertilization scheme was determined: organic fertilizers 7 500.00-12 106.88 kg/hm², nitrogen, phosphorus and potassium fertilizers 495.00-604.77 kg/hm², micronutrient fertilizers 1.92-2.76 kg/hm².