Abstract: Constituents eluted with petroleum ether of Lepidium latifolium L. were analyzed by capillary GC-MS method. The relative content of each component was calculated by area normalization and 20 constituents were separated and identified. The main chemical components were hexadecanoic (53.980%), octadecanoic acid (17.063%), docosanoic acid (4.769%), eicosanoic acid (3.363%), octacosanoic acid (2.773%), eicosanoic acid (2.719%), pentadecanoic acid (2.476%), and heptadecanoic acid (2.291%). In addition, the contents of triacontanoic acid, tetradecanoic acid, tricosanoic acid and hexacosanoic were also more than 1%. All fatty acid are saturated fatty acid.

Key words: Lepidium latifolium L., fatty acid, gas chromatography-mass spectrometry