Antifungal Lipopeptide Produced by Paenibacillus polymyxa HY96-2

doi:A

Abstract

Abstract: Isolation and purification of antifungal substance from Paenibacillus polymyxa HY96-2, a commercial biocontrol strain used in control of fusarium wilt disease was studied in this paper. By means of acidification, n-BuOH extraction, ethyl acetate precipitation, silica gel column chromatography, sephadex LH-20 column chromatography and HPLC preparation, 6B was isolated and purified. Its structure was elucidated as fusaricidin A by means of spectroscopic analysis including NMR, MS and ESI-MS/MS. Results of two-fold serial agar dilution method showed that the minimum inhibition concentrations of 6B against 15 phytopathogenic fungi 12.5~50 µg/mL. Greenhouse results showed that 6B had better control effect on Botrytis cinerea of cucumber. The control effect could reach 95% when its concentration was set at 250 μg/mL.

Key words: Paenibacillus polymyxa, antifungal activity, isolation and purification, structure identification, fusaricidin A

CLC Number:

TQ458

FAN Lei, ZHANG Dao-jing, LIU Zhen-hua, TAO Li-ming, LUO Yuan-chan, WEI Hong-gang, LI Shu-lan, LI Yuan-guang. Antifungal Lipopeptide Produced by Paenibacillus polymyxa HY96-2[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(6): 729-.

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