Studies on Transformed Panax notoginseng by Aspergillus terreus

doi:A

Abstract

Abstract: The soil fungus YM31966 was screened for transformed directly on chemical components of Panax notoginseng from Chinese traditional medicine. After identified the strain is Aspergillus terreus. It transformed Panax notoginseng products to make up main ingredients of notoginsenosides nR2, RX1 and ginsenosides Rg1, Rd, Rh1, Rh4 by solid state fermenting ways, union chemistry extraction separation method, but original compounds decomposed from Panax notoginseng such as ginsenosides Rb1, Rc, Re and notoginsenosides R1, R3, R6 and so on through high performance liquid chromatography, chromatography-mass and 1H-NMR and 13C-NMR spectrometry examinations. The result indicated that Aspergillus terreus YM31966 is a microorganisms to transform Panax notoginseng of traditional Chinese medicine, some of chemical components of Panax notoginseng from Chinese traditional medicine were remarkably changed not only to produce new compound but also to increase quantities of compound from Panax notoginseng by conversing process of Aspergillus tamarii YM31966.

Key words: Aspergillus terreus, Panax notoginseng, Transformation, Ginsenoside, Notoginsenoside

CLC Number:

Q939.9

Chen You-wei , Wu Shao-hua, Miao Cui-ping. Studies on Transformed Panax notoginseng by Aspergillus terreus[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(8): 1014-.

References

1 Zhou ZH(周志华).Analysis of Saponins Constituents from Panax Notoginseng.Act Pharm Sin(药学学报),1981,16:216-219.
2 Wei JX(魏均娴),Chen YG(陈业高).Progress on Saponins of Dammarane Type,Chin Tradit Herb Drugs(中草药),1998,25:205-208.
3 Zhao (赵平),Liu YQ(刘玉清),Yang CR(杨崇仁).Minor Costituents from the Roots of Pananx Notoginseng(1).Acta Botanica Yunnanica(云南植物研究),1993,15:409-412.
4 Chen YP(陈燕平),Meng Q(孟勤),Song CC(宋长春),et al.Preparation of 20(S)-protopanaxadiol saponins and 20(S)-ginsenoside-Rh2 from 20(S)-protopanaxadiol saponins.Chin Pharm J(中国药学杂志), 1997,32:273-275.
5 Cong DL(丛登立),Cong CC(宋长春),Xu JD(徐景达).Isolation and Identification of 20(S)-Ginsenoside-Rh1, -Rh2 and Ginsenoside –Rh3 from the leaves of panax Scinquefolium.Chin Pharm J,2000,35:82-83.
6 Qin S(秦盛),Chen YW(陈有为),Xing K(邢珂),et al.Isolation,Identification and Bioactivity Screening of Endophytic Fungus Assiciated with Opuntia ficusindica Mill.Microbiology(微生物学通报),2006,33:95-99.
7 P.M.Kirk, P.F.Cannon,J.C.David and J.A.Stalpers,Ainsworth ＆Bisby’s dictionary of the Fungi,Ninth Edition 2001,p47-48.
8 Zhang CH(张传会),Chen YW(陈有为),Zheng Y(郑毅),et al.Preparation of diosgeninby microbial transformation of dioscorea panthaica by YM32139 Strain.J Microbiol(微生物学杂志),2007,27:31-34.
9 Wang D(王东),Li HZ(李海舟),Chen KK(陈可可),et al.HPLC comparative analysis of ginsenoside saponins in different underground parts of panax notoginseng.Acta Botanica Yunnanica(云南植物研究), 2005,6:287-289.
10 Li HZ(李海舟),Zhang YJ(张颖君),Yang CR(杨崇仁).A Further Investigation on the Chemical Constituents from the Leaves of Panax notoginseng.Nat Prod Res Dev(天然产物研究与开发),2006,4:131-233.
11 Zeng J(曾江),Cui XM(崔秀明),zhou JM(周家明),et al.Studies on Chemical Constituents from Rhizomes of Panax notoginseng.J Chin Med Mat(中药材),2007,1:7-9.
12 Yuan YQ(袁延强),Chen XQ(陈锡强),Hou HR(侯海荣),et al.Research on Dammarane Saponins of Panax notoginseng (Burk.) F.H.Chen(Ⅱ).Shandong Science(山东科学),2009,4:276-279.
13 Zhou J.Dammarane-saponins of Sanchi-Ginseng,roots of Panax notoginseng(Buek.)F.H.Chen:Structure of new saponins, notoginsenoside-R1 and–R2 and identification of Ginsenoside-Rg2 and–Rh1.Chem Pharm Bull. 1981,29(10):2844.
14 Kazuhiro Hirakura.Phytochemistry.1992,31(3):889-903.
15 Kazuhiro Hirakura.Phytochemistry.1993,35(4):963-967.
16 Masayaki Yoshikawa,Chem Pharm Bull.1998,46(4):647-654.
17 Hideaki M,Kensuke N,Seiya F,et al.Effects of Red Ginseng on Experimental Disseminated Intravasular Coagulation.Chem Pharm Bull,1986,34:2100-2104.