Abstract: Twelve compounds were separated and purified from the ethyl acetate extracts in the alcohol solution of Polygonatum rhizome by silica gel and Sephadex LH-20 column chromatography,respectively.Their structures were identified as ningpogenin (1), 6-O-p-hydroxybenzoylaucubin (2); 3,3′-Bisdemethylpinoresinol (3); 5-Hydroxymethyl-2-furancarboxaldehyde (4), (22E,24R)-Ergosta-7,22-dien-3β-ol (5), Borreriagenin (6), Cerevisterol (7); 5α,8α-epidiory-(22E,24R)-ergosta-6,22-dien-3β-ol (8), β-sitosterol (9 ), 3β,5α,9α-trihydroxyergosta-7,22-dien-6-one (10), ergosta-7,22-dien-3-one (11), p-hydroxybenzylethanol (12). All the compounds were isolated from the plant for the first time,except the compound (9).

Key words: Polygonatum odoratum, extraction, isolation, structure identification