NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (5): 726-730.

• Article • Previous Articles     Next Articles

Optimization of Aqueous Two-Phase Extraction of Saponins from Rubus parvifolius L.by Modified Central Composite Design and Response Surface Methodology

LI Na1, ZHOU Wen-jie1,2, YU Qi-nan1,2, YANG Rong-ping1,2*   

  1. 1 Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China; 2 Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China
  • Online:2014-05-31 Published:2014-11-17

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Abstract: The aqueous two-phase of ethanol/ammonium sulfate system was used for the extraction of saponins from Rubus parvifolius L..The extraction conditions were optimized using modified central composite design coupled with response surface methodology with two independent variables including quantity of (NH4)2SO4 and CH3COOH,and the saponin content as the dependent variable.The optimalconditions were determined as follows:12.14% (NH4)2SO4 and 40.00% CH3COOH. The extraction yield of saponins was 2.95%.The results showed that the central composite design and response surface methodology was useful for optimizing the aqueous two-phase extraction of saponins from R.parvifolius.The developed extraction technology was simple,energy-saving and easy to zoom in industrial production.

Key words: Rubus parvifolius L., saponin, central composite design and response surface methodology, aqueous two-phase system

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