Abstract: Using Dictyophora echinovolvata pileus as the raw material,the optimal extraction process of protein was studied by single factor and orthogonal experiments.The molecular weight distribution of extracted protein was analyzed by SDS-PAGE.The results indicated that the pH and extraction time were the most important factors to the extraction process.The highest extraction yield of 64.07% was obtained at pH 8.5,55 ℃ with 2.5 h of extraction.The major molecular weights of extracted protein were 16 kDa and 38 kDa.In conclusion, D.echinovolvata pileus is a suitable raw material for extracting proteins with great economic potential and market prospect.

Key words: Dictyophora echinovolvata pileus, protein, extraction