Abstract: The objective of this study was to investigate the anti-cancer activity of petroleum ether extracts of Fuscoporia punctata (PEFP) and its in vitro anti-cancer mechanism. The sulforhodamine B (SRB) assay was employed to test the antitumor effect of PEFP on SMCC-7721,SGC-7901 and Hep-2 cell lines.Inverted phase contrast microscope and transmission electron microscopy were used to observe the changes of morphologic and ultrastructure of SMCC-7721 cells after treatment with PEFP.The changes of SMCC-7721 cells cycle were analyzed by flow cytometry (FCM).The results showed PEFP displayed obvious inhibitory effect on proliferation of the above-mentioned cells in a dose-dependent manner.The IC₅₀ values were 52.72 μg/mL,69.18 μg/mL and 58.88 μg/mL respectively.After treatment with PEFP at the 1/2 IC₅₀ concentration for 48 h,the morphology and cell cycle of SMCC-7721 cells showed special changes.The cell cycle analysis revealed that SMCC-7721 cells increased in G₀/G₁ phase and decreased in S-phase cells.The SMCC-7721 cells were arrested in G₀/G₁ phase.The study indicated PEFP can inhibit SMMC-7721 cells proliferation by blocking the cell cycle to G₀/G₁ phase and had relatively obvious in vitro anti-cancer effect.

Key words: Fuscoporia punctata (Fr.) Cunn, anti-cancer, cell proliferation, cell cycle