Abstract: The objective of this study was to optimize the ultrasonic-assisted extraction of total triterpenoid from the root of Actinidia eriantha. Four factors,namely ethanol concentration,ratio of solvent to material,ultrasonic power and extraction time,were selected for the method optimization.Based on single factor experiments,the conditions of ultrasonic-assisted extraction for total triterpenoid from the root of A.eriantha was optimized through response surface analysis (RSA) with four factors and three levels.The optimal extraction conditions were obtained as follows:72% as ethanol concentration,1:20 g/mL as material-liquid ratio,400 W as ultrasonic power,45 min as extraction time.Under these conditions,the extraction yield of triterpenoid was determined to be 12.65 mg/g in the confirmatory experiment,which was close to the theoretical value.Hence,the ultrasonic extraction of triterpenoid extracted from the root of A.eriantha was successfully optimized by RSA.

Key words: Actinidia eriantha Benth., triterpenoid, ultrasonic extraction, response surface analysis