Abstract: Fourteen compounds were isolated and purified from the roots of Nothapodytes pittosporoides by column chromatography on silica gel,MCI and Sephadex LH-20.Their structures were elucidated as β-sitosterol(1),β-daucosterol(2), 7-oxo-β-sitosterol(3), β-sitosteryl-3-O-β-D-glucopyranoside-2′-O-palmitate(4), 5α,6β-dihydroxydaucosterol(5),lupeol(6),3-acetoxy-12-oleanen-28-ol(7),camptothecin(8),9-methoxycamptothecin(9),10-hydroxy camptothecin(10),9-methoxymappicine-20-O-β-D-glucopyranoside(11),mappicine-20-O-β-D-glucopyranoside(12),(3S)-pumiloside(13), (3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(14).Compounds 1-7,9-14 were obtained from this plant for the first time．

Key words: Nothapodytes pittosporoides, root, chemical constituent, camptothecins