NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (2): 230-232.

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Simultaneous Determination of Apigenin and Quercetin in External Application Liquid of Potentilla kleiniana Wight et Arn by HPLC

DONG Ai-wen1*, ZHU Chun-yan2, ZHUO Qi3   

  1. 1 Key Laboratory of Forest Products Chemical Engineering of Hunan Province,Jishou University,Zhangjiajie 427000,China; 2 College of Biology and Environmental Sciences,Jishou University,Jishou 416000,China; 3 College of Urban-rural Resources and Planning Science,Jishou University,Zhangjiajie 427000,China
  • Online:2014-02-28 Published:2014-12-12

Abstract: The objective of this study was to establish a HPLC method for the simultaneous determination of apigenin and quercetin in the external application liquid of Potentilla kleiniana Wight et Arn.Reversed phase chromatographic column Shim-pack VP-ODS C18 (4.6×150 mm,5 μm) was used for the separation of apigenin and quercetin under the conditions of methanol-4% phosphate acid(50:50,v:v) as the mobile phases,column temperature of 30 ℃,flow rate of 1.0 mL/min,detection wavelength at 360 nm and injection volume of 10 μL.The results indicated that both apigenin and quercetin had good linear relationship in the range of 60-300 μg/mL with correlation coefficients of 0.9996 and 0.9997 respectively.The contents of apigenin and quercetin of the alcohol extract of P.kleiniana were higher than that of water-decocted liquid extract.In conclusion,the HPLC method developed in this study was convenient,quick and accurate.It can be used for determination of apigenin and quercetin in P.kleiniana.

Key words: Potentilla kleiniana Wight et Arn, apigenin, quercetin, external application liquid, HPLC

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