NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (5): 854-858.doi: 10.16333/j.1001-6880.2015.05.020

• Article • Previous Articles     Next Articles

Isolation and Purification of Flavones from Polygonum aviculare L.by Polyamide Chromatography Combined with High-Speed Counter-Current Chromatography

SUN Zhao-lin1,2 , WANG Xiao1,2, WANG Dai-jie2, LI Jia1, DUAN Wen-juan2*   

  1. 1 Shandong University of Traditional Chinese Medicine,Jinan 250355,China; 2 Shandong Analysis and Test  Center,Key Laboratory for Applied Technology of Sophisticated Analytical Instrument of Shandong Province,Jinan 250014,China
  • Online:2015-05-30 Published:2015-06-05

Abstract: In this study,three flavones were isolated and purified from Polygonum aviculare L.by polyamide chromatography combined with high-speed counter-current chromatography (HSCCC). A rapid and efficient method was established for preparative isolation and purification of three flavonoids from P.aviculare. The crude extract of P.aviculare was objected to polyamide chromatography to afford the flavones-enriching fraction.After that,the fraction was further separated by HSCCC with two phase solvent systems composed of ethyl acetate-methyl alcohol-water-formic acid (4:1:5:0.1,v/v).The lower phase as the mobile phase was operated at a flow rate of 2.0 mL/min,while the apparatus rotated at 850 rpm and the detection wavelength was 254 nm.As a result,7.5 mg of myricitrin,13.8 mg of astragalin,20.6 mg of desmanthin-1 were purified from 150 mg of the flavones-enriching fraction and each with 94.86%,94.28% and 91.86% purity as determined by HPLC.These compounds were identified by mass spectrometry (MS) and 1H-nuclear magnetic resonance (1H NMR).The method was simple,rapid and suitable for the preparative separation of flavonoids.

Key words: Polygonum aviculare L., high-speed counter-current chromatography;polyamide chromatography;myricitrin;astragalin;desmanthin-1

CLC Number: