NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (7): 1205-1209. doi: 10.16333/j.1001-6880.2015.07.015

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Separation of High-Polarity Peptides Using Two dimensional Preparative HPLC

JIANG Lei1,4,ZHAO Huan2,TAO Yan-duo1,WANG Shuo1,4,SHAO Yun1,WANG Qi-lan1,ZHANG Yao-zhou3*,MEI Li-juan1*   

  1. 1 Key laboratory of Tibetan medicine research,Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xining 810001,China; 2 Harmonia Biotechnology (Tianjin) Limited Company,Tianjin University;  3 School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China;  4 University of Chinese Academy of Sciences,Beijing 100039,China
  • Online:2015-07-20 Published:2015-07-23

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Abstract: In the present separation work,Osteon Myospalacem Baileyi water extracts were taken as an example to explain the purification method.The first dimensional preparation was carried out on a C18 AQ prep column to obtain fractions from the crude sample.A C18 MP prep column was used for the second dimensional preparation to purify single compounds from the fractions.The purities of all the compounds isolated from the fractions were higher than 98%,which indicated that the developed method was efficient to isolate single compounds with high purity from the extract of Osteon Myospalacem Baileyi.After structural identification by UPLC-LTQ-MS/MS,the sequences of the purified peptides were determined to be KTAILVKE,RGAPQDQE,LVGPGAPGR,GFAGD and KPQWHP,respectively.The developed method showed great potential in the purification of traditional animal medicines.

Key words: high performance liquid chromatography, Osteon Myospalacem Baileyi, peptides, two-dimensional separation

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