Abstract: This study was designed to investigate the protective effects of a new chalcone derivative G01,3′-formyl-4′,6′-dihydroxy-2′-methoxy-5′-methyl-3,4-dihydroxy chalcone,on H₂O₂-induced oxidative damage in cortical neurons of mice,and the underlying mechanisms.Primary cortical neurons of newborn mice were cultured in Neurobasal containing B-27,free of serum,and the oxidative damage was induced by 50 μmol/L H₂O₂. After the damage,the effects of different concentrations of G01 (0.001,0.01,0.1 g/L) on cell survival was tested by MTT assay,and the levels of LDH,MDA and SOD were measured by biochemistry assay.Compared with the damage group,0.01,0.1 g/L G01 significantly improved the survival rate of neurons to 74.51% and 81.31%,respectively,reduced the release of LDH in the medium,inhibited MDA synthesis,and enhanced the activity of SOD.These results indicated G01 had significantly protective effects against H₂O₂-induced damage in cortical neurons,and the mechanisms were related to its antioxidative effects.

Key words: chalcone analogues, neurons of mice, oxidative damage, antioxidation, neuroprotective effect