天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (1): 11-17.doi: 10.16333/j.1001-6880.2015.01.002

• 研究论文 • 上一篇    下一篇

C18硅胶制备荔枝果皮原花青素低聚体的组成分析及单体分离

李书艺1,2，吴茜2，隋勇2，孙智达2*   

  1. 1 武汉轻工大学食品科学与工程学院，武汉 430023；2 华中农业大学食品科学技术学院 环境食品学教育部重点实验室，武汉 430070
  • 出版日期:2015-01-31 发布日期:2015-02-12

Purification and Analysis of Litchi Pericarp Oligomeric Procyanidins

LI Shu-yi1,2, WU Qian2, SUI Yong2, SUN Zhi-da2*   

  1. 1 College of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China; 2 Key Laboratory  of Environment Correlative Dietology, College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Online:2015-01-31 Published:2015-02-12

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摘要： 为更快速地从荔枝果皮中分离制备纯度更高的A-型原花青素低聚体，本研究在传统大孔树脂富集纯化的基础上，利用C18硅胶填料对制备方法进行了优化。通过组分分析和HPLC、LC-MS鉴定可知，新法制得的荔枝果皮原花青素低聚体（Litchi pericarp oligomeric procyanidins，LPOPC）总酚含量高达940±123 mg/g，且主要为(-)-表儿茶素、A-型原花青素二聚体和三聚体，相对含量分别占44.86%、36.02%和9.86%，平均聚合度约为1.62。因此，当再次通过Toyopearl HW-40s凝胶对提取物进行柱层析分级时，可得到纯度较高的已知A-型原花青素二聚体和三聚体。等辐射分析法（Isobologram）研究显示，该A-型二聚体和三聚体均可清除自由基，并呈现出协同抗氧化性。

关键词: 荔枝果皮, A-型原花青素低聚体, C18硅胶, 抗氧化

Abstract: In order to obtain Atype oligomeric procyanidins from litchi pericarp with higher purity,C18 silica gel column was used to optimize the traditional preparation process,which was purified and enriched by using AB-8 macroporous resin.According to the component analysis,HPLC and LC-MS identification of litchi pericarp oligomeric procyanidins (LPOPC) prepared by C18 silica gel,the total phenol content of LPOPC was up to 940±123 mg/g,in which (-)-epicatechin monomer,A-type procyanidin dimer and trimer played an important role,occupying 44.86%,36.02% and 9.86%,respectively.The average polymerization degree of the new LPOPC was about 1.62.Consequently,the known A-type procyanidin oligomers,such as procyanidin A2 and trimer,epicatechin (4β→8,2β→O→7)-epicatechin-(4β→8)-epicatechin,can be isolated from the LPOPC more efficiently by Toyopearl HW-40s column.In addition,the isobologram analysis suggested that the purified A-type procyanidin dimer and trimer all possessed strong radical scavenging capacities,and showed synergistic antioxidant effect.

Key words: litchi pericarp, A-type procyanidin oligomer, C18 silica gel, antioxidant

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TS264.4

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